FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n02_E2W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n02_E2W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24263046
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT24850.013.2295562
GCGTGAC37750.09.3656771
AATTGAC41850.08.9277955
GTGACTC40200.08.9046963
GTACTCG47700.08.7309784
GAATTGA62050.08.5620894
GTAGAAC28050.08.50057911
CTCTATA61150.08.46032845
GGAAAAT97400.08.1846631
AATCGAA46250.08.0784485
AAAATCC73800.08.0537023
GAATCGA33650.08.0279684
GCTCTAG46500.07.885153311
CACTCGT62650.07.827667735
GCGGAAA75950.07.7980221
TACTCGA51950.07.71198275
ACTCGTG62950.07.64748536
AGTACTC56550.07.5643063
TCAGGTA36150.07.46739347
AGAACGG148600.07.4389353
Produced by FastQC (version 0.11.5)