FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n02_E2W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n02_E2W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25342742
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC30650.09.61408711
AATCGAA48750.09.5111755
GTACTCG49650.08.9779034
CGTGACT23400.08.8533462
GAATTGA66900.08.5474514
CGTAGGT14750.08.38813335
CTCGAAT22750.08.3151681
TACTCGA51950.08.2754085
GAATCGA36350.08.1752034
GGAAAAT111550.08.0350141
AGAACGG149200.07.9676953
AAATCGA51250.07.95085334
GGGAAAT53350.07.8936951
TCAGGTA42750.07.84050277
AGTACTC60500.07.7778323
AATTGAC43750.07.66566325
GGGGAAT47350.07.65736061
GCGTGAC32200.07.4134981
AATTGAA71000.07.3865075
AAGAACG178600.07.3632042
Produced by FastQC (version 0.11.5)