FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n02_E2W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n02_E2W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23846806
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT24000.011.3529072
GTAGAAC28000.011.24701511
GTACTCG49550.010.3132974
GCGTGAC30000.010.0588731
TACTCGA54650.09.1841295
TCAGGTA38000.09.0574157
AGTACTC59650.08.9076553
GGAAAAT93350.08.6846831
GAATCGA35800.08.3634214
CTCGAAT19600.08.1577071
TCGGGGG40550.08.10023439
GAATTGA61150.08.0623754
GGTAGAA39350.08.06024210
GTGACTC38550.08.0596573
AGAACGG151400.07.9856493
AATCGAA45800.07.96109535
CAGGTAG42550.07.8773718
TAGAACA41350.07.83363612
ACAATCG19300.07.58259533
AAAATCC63500.07.55209973
Produced by FastQC (version 0.11.5)