FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n02_E2W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n02_E2W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28075672
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT29350.011.0478872
GTAGAAC31100.010.27071611
GTACTCG58100.09.4930964
GGGGGGC214950.09.18800643
GCGTGAC39300.09.1682631
TACTCGA61300.08.885655
GGAAAAT117800.08.5834261
AGTACTC67600.08.526123
TCAGGTA42750.08.52463157
AATCGAA45900.08.434325
TCGGGGG57450.08.30185339
GGGAAAT58950.08.2514371
GGGGGCC88250.08.15618644
AATTGAC47600.07.84938145
GAATCGA38750.07.84296044
AAAATCC87600.07.81317763
CTCGAAT22800.07.80282551
TATAAGG32150.07.70098075
ATGTCTC102550.07.611286643
GTGACTC45850.07.61115553
Produced by FastQC (version 0.11.5)