FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n02_E2W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n02_E2W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23158108
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23700.013.20714952
GTACTCG50950.010.6931094
GCGTGAC32700.010.4682741
GTAGAAC26050.010.4482511
TACTCGA57350.09.4191985
GTGACTC36250.09.2546063
AGTACTC62400.08.9484293
CTCGAAT20450.08.1492511
AATTGAC38800.08.0634185
GAATCGA34650.08.056594
GAATTGA58250.07.96166854
TCAGGTA39600.07.95289047
GGGAAAT47600.07.94843341
GGAAAAT99300.07.86972331
GCTCTAG46050.07.76664411
CTCTATA52600.07.65450745
AAAATCC76500.07.59342963
TATCCAG56500.07.4495375
ATGTCTC94100.07.434290443
ATTGACC37900.07.4193976
Produced by FastQC (version 0.11.5)