FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18444811
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA6900.032.5877916
ACGTCTG7200.031.22962815
CACGTCT7800.030.26338414
CACACGT8150.027.86042412
ACACGTC8750.026.72071513
CAGTCAC6400.026.05902327
AGCACAC9700.022.9408610
CCAGTCA7900.022.82300626
ACCGATC6250.022.30466534
GAGACCG6600.021.84905431
CCGAGAC6650.021.69025829
GCACACG10600.021.63589111
TCGGAAG16950.019.8982013
CTCCAGT10000.019.60349724
ATCGGAA17400.019.5139272
AACTCCA11200.018.89919322
CGAGACC7650.018.85267430
GATCGGA18400.018.3361531
AGACCGA7950.018.13361432
CGGAAGA18500.018.1087274
Produced by FastQC (version 0.11.5)