FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n01_E2W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n01_E2W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27038120
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA293280.10846907995082498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC34600.022.36894838
ACGTCTG36650.022.28393415
CCGTACG37200.021.34985733
CGTCTGA39300.020.78132816
TCGTATG36300.019.39989345
ACCGTAC44850.018.66134332
CTCGTAT38950.018.36878644
CACCGTA44900.017.93936731
TCGGAAG53000.015.4552883
ATCGGAA54550.015.0171922
CACGTCT56600.014.82694314
CACACGT61150.014.05497612
GTAATCT58800.013.77489839
CAGTCAC62350.013.4966927
TACGTAA59100.013.28621236
ACGTAAT61050.012.7881337
AATCTCG62900.012.44770941
AGTCACC74800.011.55105828
TAATCTC71350.011.50958640
ACACGTC75000.011.42938713
Produced by FastQC (version 0.11.5)