FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n01_E2W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n01_E2W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26738950
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG33800.028.68973433
GTTTCGG39600.024.6580134
AGTCACG41700.023.30985628
ACGTCTG42150.023.2726115
TTTCGGA44750.021.71971935
ACGTTTC47150.020.7573132
TTCGGAA46900.020.7240436
CGTCTGA47800.020.52176916
GTCACGT51600.018.92479329
CTCGTAT41400.018.4772144
TCGTATG42350.017.42528345
ATCGGAA58350.017.163962
TCGGAAT58200.017.0868837
TCGGAAG60850.016.6422523
CACACGT64050.015.77199612
CGGAATC62800.015.512856538
TATGGGT38900.015.21121843
CACGTCT67450.015.11027714
GGAATCT67550.014.68847639
ACACGTC71750.014.48692713
Produced by FastQC (version 0.11.5)