FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n01_E2W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n01_E2W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28374358
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG59600.026.50006515
AGTCACG59650.025.98976528
TCGTATG55850.025.01651445
CACGTGG63100.024.53185831
CGTCTGA64950.024.45578616
CTCGTAT59000.024.2147144
GGCCTTA66350.023.22836
GTCACGT68300.022.76408429
CACGTCT78250.020.47159614
GCCTTAT74350.020.45633937
TCGGAAG79050.020.240813
TATCTCG73600.020.20611241
ATCGGAA80500.019.7379442
ACGTGGC78800.019.70116632
CCTTATC79550.019.2605738
CACACGT86050.018.48535212
CTTATCT85750.018.02536239
ACACGTC94450.017.5796613
CAGTCAC91250.017.40866527
TCACGTG93800.016.57549330
Produced by FastQC (version 0.11.5)