FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n01_E2W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n01_E2W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24105919
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA344770.1430229646088166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG37950.023.891915
AGTCACG37750.023.24614728
CGTCTGA39950.022.41422316
TCGTATG38950.021.9499545
CGTGAAA41100.021.4587933
ACGTGAA44500.020.42603932
AACGATC41350.020.18638438
CTCGTAT40700.019.9005744
CACGTGA45750.019.5241931
GTCACGT48250.018.65374629
TGAAACG48650.018.22105235
TCGGAAG50350.018.1012823
CACGTCT53700.017.09398514
GAAACGA52300.017.07846836
AAACGAT52350.016.76131437
ATCGGAA54650.016.4725062
CACACGT63600.014.6454412
CAGTCAC62900.014.34488627
TCACGTG68250.013.55004530
ACACGTC73500.012.825788513
Produced by FastQC (version 0.11.5)