FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n01_E2W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n01_E2W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24263046
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG545060.2246461553096013TruSeq Adapter, Index 18 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357760.14745057153994598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG83450.033.56851228
ACGTCTG84650.033.43598615
ACGTCCG85450.032.85985632
CGTCTGA86400.032.83687216
GTCCGCA86250.032.45092434
TCGTATG82550.032.24245545
CTCGTAT85500.031.5510344
GTCACGT93650.030.05651529
TCGGAAG99600.028.8752023
CCGCACA97700.028.48662236
CACGTCT99900.028.46702214
ATCGGAA101250.028.339662
TCCGCAC100850.027.8199535
TCACGTC103100.027.49793430
CACACGT107700.026.67708212
CAGTCAC108100.026.18448627
ACACGTC115700.025.02681713
CGCACAT111500.024.65824537
TCTCGTA114400.023.77719943
CCAGTCA120650.023.68456826
Produced by FastQC (version 0.11.5)