FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n01_E2W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n01_E2W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25342742
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTATG482490.1903858706370447TruSeq Adapter, Index 16 (97% over 39bp)
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA304430.1201251230036592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG85950.033.53192515
GTCCGCA85150.032.4732636
CGTCTGA90400.032.1301716
TCGTATG73450.030.96816345
CTCGTAT77100.030.93190844
TCGGAAG101700.028.5890433
CACGTCT103150.028.3331714
TCCGCAT97650.028.03993437
ATCGGAA104300.027.9214532
CGCATCT98600.027.5415939
ACCCGTC102700.027.29683132
TCACCCG110300.025.54020130
CACACGT113900.025.40246812
CAGTCAC113100.025.00762627
ACACGTC117750.024.76277413
GTCACCC114850.024.50902729
CCGTCCG117800.024.2749834
CCCGTCC116500.024.08260533
AGTCACC118700.024.05529428
CGTCCGC120750.023.53286735
Produced by FastQC (version 0.11.5)