FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n01_E2W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n01_E2W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23846806
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG622740.26114189044855735TruSeq Adapter, Index 15 (97% over 40bp)
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA239950.10062144171424886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG87400.033.69656445
CTCGTAT98550.032.85184544
GTCAGAA172750.030.1505936
CGTCTGA177850.029.5384516
ACGTCTG177600.029.52935815
AATCTCG141500.029.46289841
TGTCAGA179350.029.20414435
GTCACAT185700.028.09894229
AGTCACA189700.027.53023128
CAGTCAC190650.027.40485227
GAATCTC184300.027.2962540
CACGTCT193750.027.16083714
ACATGTC196450.026.70770832
ATGTCAG198200.026.50611534
AGAATCT192500.026.28582439
TCGGAAG201300.026.2374383
CACACGT202300.026.23561712
CCAGTCA198800.026.2247726
TCTCGTA128000.026.0843243
ATCGGAA203400.025.9354272
Produced by FastQC (version 0.11.5)