FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n01_E2W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n01_E2W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28075672
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG2098500.7474442642014054TruSeq Adapter, Index 14 (97% over 44bp)
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA337870.12034262260935374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG245650.039.9052445
CCGTATC301350.037.89734338
CTCGTAT269950.037.72141644
TCCGTAT303600.037.67576637
CGTATCT300450.037.6738939
AGTTCCG315500.036.732534
TTCCGTA314450.036.5260336
GTTCCGT321200.035.856535
TATCTCG305800.035.6461941
TCTCGTA298650.034.3525643
GTATCTC330100.034.31029540
ACAGTTC351400.033.02485732
ACGTCTG358100.032.9720215
CGTCTGA359800.032.75995316
GTCACAG355050.032.68803429
TCACAGT359850.032.17049830
CACAGTT361050.032.1616831
CAGTCAC365250.031.89839627
AGTCACA365750.031.7748128
CACGTCT379500.031.1364414
Produced by FastQC (version 0.11.5)