FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l05_n01_E2W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l05_n01_E2W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23158108
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA272690.11775141561650891No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262410.11331236558703328No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG237550.10257746444571379TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG48700.027.62659515
CGTCTGA49300.027.33600416
TCGTATG46000.026.36275345
CTCGTAT46150.025.98444644
TCGGAAG62600.021.821233
ATCGGAA63050.021.738612
CACGTCT66250.020.71567514
AGTCAAC64350.020.69830934
CACAGTC65950.020.5037231
GTCACAG67950.019.73573129
CACACGT69850.019.61592712
AATCTCG66450.019.19751741
CAGTCAC71100.019.11453427
TCACAGT73600.018.3734330
AGTCACA73850.018.25041428
ACACGTC79550.017.56331813
TCTCGTA72800.017.1521943
ACAGTCA82800.016.6024332
CCAGTCA84700.016.17820426
AGCACAC91000.015.65021410
Produced by FastQC (version 0.11.5)