FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16336776
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT8050.029.3763312
AGTGTAG4350.027.9348529
GTAGATC4450.027.3052632
GAGTGTA4750.025.58244328
GTGTAGA4750.025.58228730
TCTCGGT4850.025.05128537
TAGATCT4950.024.54632433
GTGTAGG5800.021.72104817
TGTAGGG5850.021.5345418
TCGTGTA6100.021.39000515
TGTAGAT5850.020.7708631
AGCGGTT5600.020.48495510
TAGGGAA6250.020.15639120
CTCGGTG6500.019.73237638
GTAGGGA6500.019.03499419
GATCTCG7100.018.69555335
TCGGTGG6850.018.39633839
AGATCTC6850.018.39289734
TTCCCTA4700.018.1788188
TTTCCCT7300.017.247147
Produced by FastQC (version 0.11.5)