FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n02_D2W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n02_D2W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27284955
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26350.010.2566282
GTACTCG55900.09.4664574
GTAGAAC32650.09.23215611
TACTCGA55150.09.1840925
GCGTGAC36300.08.6875461
GGAAAAT120050.08.4998591
AAAATCC87150.07.85556553
AGTACTC68900.07.81177953
GAATTGA76800.07.76991224
CACTCGT51150.07.74083135
GAATCGA42450.07.69169044
AATCGAA55100.07.68078235
ACTCGTG52550.07.663232336
CTCTATA54900.07.539934645
GCGGAAA64350.07.42100951
CTCGAAT23250.07.26631071
TCAGGTA48350.07.25755837
AATTCAC44550.07.17529635
CGGAAAT24500.07.17140441
AATTGAC48350.07.17007065
Produced by FastQC (version 0.11.5)