FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n02_D2W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n02_D2W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26859096
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGA39400.012.8014864
CTAATCG36900.012.3275653
GTAGAAC48200.011.38713711
AATCGAG61700.010.580355
TCAGGTA58050.010.1132667
CAGGTAG60150.09.5357958
TAGAACA62400.08.90387912
GGTAGAA65150.08.73499110
AAAATCC103200.08.4228663
AGGTAGA67300.08.3890639
GCTAATC61950.08.3612342
GGAAAAT142800.08.3127361
GAATCGA41450.07.71387154
GAATTGA71550.07.6787194
ATCAGGT109900.07.63488156
CCCGAAT19200.07.6255941
GGGAAAT69650.07.53522731
AAATCCA100150.07.5093814
AAATCCT108100.07.3528824
CTCGAAT29450.07.342561
Produced by FastQC (version 0.11.5)