FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n02_D2W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n02_D2W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30346387
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC40800.010.69601211
CGTGACT26500.010.3684052
TCAGGTA51050.09.2531347
GTACTCG54550.08.7924484
GCGTGAC36700.08.4086091
TACTCGA57050.07.9310895
AATCGAA55650.07.928365
GGAAAAT120900.07.8624121
GCGGAAA81550.07.84447771
TAGAACA59150.07.719987412
CAGGTAG59050.07.6566978
GAATTGA84700.07.6033954
GGTAGAA58400.07.51087710
CACTCGT67050.07.3814735
AACGGCC108900.07.37959965
CTCGAAT27400.07.31659651
AGAACGG173150.07.29633433
GAATCGA47100.07.26687344
ACTCGTG70950.07.261213336
AGTACTC73600.07.1904223
Produced by FastQC (version 0.11.5)