FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n02_D2W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n02_D2W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16949537
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC23300.010.52325511
GTACTCG29850.09.9576134
CGTGACT14550.09.5969642
TCAGGTA28700.09.1698397
TACTCGA31400.09.0331315
GGTAGAA29350.08.4304110
AAAATCC48700.08.36994653
AGTACTC38150.08.2052793
TATAAGG19500.08.1963625
GGAAAAT67400.08.187981
GAATCGA26450.08.0876734
CTATAAG18350.07.97632654
AATCGAA27100.07.89135745
GAATTGA44750.07.6484984
AGAACGG92850.07.6401473
CAGGTAG31800.07.63931478
AATTGAC27550.07.51733065
TAGAACA32850.07.39535312
CCGGTAT8259.094947E-127.37191631
ATATCCA44350.07.3620714
Produced by FastQC (version 0.11.5)