FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n02_D2W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n02_D2W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29811680
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT31150.014.1705752
GCGTGAC42850.011.3544871
GTACTCG72200.010.8220864
GTAGAAC44400.010.7913711
TACTCGA73650.010.5139145
GTGACTC48650.010.1372323
TCAGGTA53700.09.8436657
AGTACTC85750.09.66433053
CTATAAG33500.08.9397544
GGAAAAT132000.08.9246741
TATAAGG35150.08.7735225
GAATCGA46100.08.7432084
AATTGAC48600.08.5686855
AAAATCC99700.08.5153853
CTCGAAT28350.08.4220421
AATCGAA59550.08.3160995
GGTAGAA59700.08.21393410
CTAGCAC58250.08.1867714
TAGAACA58550.08.183310512
GCTCTAG62750.07.994127811
Produced by FastQC (version 0.11.5)