FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n02_D2W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n02_D2W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27920638
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC37800.011.48545311
CGTGACT25450.010.7963092
GAATCGA42950.09.4370694
TCAGGTA51200.09.2698637
GTACTCG56300.08.8391724
GGAAAAT132800.08.7354111
GGTAGAA52950.08.70881110
GCGTGAC35350.08.4112141
TACTCGA57100.08.3972855
GAATTGA76150.08.2797244
TAGAACA54600.08.11619212
AAAATCC91950.07.9597153
CAGGTAG55550.07.89607678
AATCGAA57950.07.73027665
AGAACGG151100.07.6904393
CCGAAAT26800.07.5645291
AGTACTC69250.07.4795243
ATGTCTC119800.07.45539643
TTAAGTC19800.07.39352372
AATTGAG111400.07.3756975
Produced by FastQC (version 0.11.5)