FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n02_D2W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n02_D2W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25922472
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT22350.011.1851612
GTAGAAC32500.010.24368611
TCAGGTA44050.08.7828127
GTACTCG54450.08.5603124
GCGTGAC33050.08.5193041
GAATCGA41200.08.471334
GGAAAAT111900.08.4544391
AAAATCC78850.08.1395853
AATCGAA49300.08.1274825
TACTCGA57100.08.0422395
GAATTGA68050.08.0076374
GGTAGAA43100.07.985037310
AATTGAC44650.07.66311075
TATAAGG29900.07.6038435
AGTACTC69150.07.58792073
AATTCAC40700.07.57720665
TAGAACA46250.07.53866612
GAAAAAT124050.07.48110961
CAGGTAG49600.07.3919028
AAAAATC71700.07.3501042
Produced by FastQC (version 0.11.5)