FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n02_D2W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n02_D2W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31377657
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT28200.010.4623952
GTAGAAC35550.09.74451411
CTCGAAT26950.09.0270381
AAAATCC100650.08.7485873
GGAAAAT147050.08.6089771
AATTGAC56650.08.5037015
GTACTCG64000.08.4089874
CTATAAG32500.08.31426054
GCGTGAC39050.08.19121
AATCGAA58150.08.0907855
GAATTGA85500.08.0589984
TACTCGA65200.07.90645845
ATTGACC51700.07.83196546
GGGAAAT68800.07.7923561
TCAGGTA49250.07.7641667
TATAAGG37150.07.7561335
GAATCGA44450.07.54814534
AAATCCT109900.07.41714244
AGTTACG7454.4201443E-107.2467586
AATTCAC47550.07.14859255
Produced by FastQC (version 0.11.5)