FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n02_D2W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n02_D2W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24269940
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23500.011.4045772
GTAGAAC28150.011.18731611
GTACTCG54700.010.5384024
TACTCGA55400.010.1585645
GCGTGAC32500.09.5646531
GAATCGA35050.09.4439014
AGTACTC64250.09.4285953
TCAGGTA38450.09.0675347
CTCGAAT22900.08.9511621
AATCGAA45750.08.8569365
GGAAAAT108200.08.6187951
GAATTGA63600.08.1431584
AAAATCC76400.08.0470573
ATGTCTC99950.07.94554443
TGTCTCC103000.07.819329344
AATTGAC38200.07.7788015
TAGAACA42250.07.719941612
GATTCGA106550.07.474474434
GTGACTC40100.07.4130573
TATAAGG25250.07.399785
Produced by FastQC (version 0.11.5)