FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16336776
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG4750.033.60574315
CGTCTGA4800.032.3262316
CACGTCT5500.029.43137714
CACACGT5700.028.39869912
ACACGTC6000.027.35338613
CCGAGAC4750.025.1600129
AGACCGA4950.024.12292332
AGCACAC7350.022.93220910
CCAGTCA5400.022.54913526
GAGACCG5350.022.32105331
ACCGATC5250.022.2814934
CAGTCAC5550.019.90824527
GCACACG8400.019.53520211
CTCCAGT7850.018.38208824
TCGGAAG12900.018.2968043
CCGATCT6650.018.26475135
AGCGGTT7100.018.0464111
CGAGACC6800.017.90446530
GGAATGC7050.017.89680523
AGTACGG1153.4816567E-617.5941451
Produced by FastQC (version 0.11.5)