FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n01_D2W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n01_D2W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27284955
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA358670.13145339620314567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC35050.021.82639538
ACGTCTG37300.021.47318515
CCGTACG38200.020.79141833
CGTCTGA39600.020.22600616
ACCGTAC41650.019.1769632
CTCGTAT39300.018.8356644
TCGTATG41100.017.6822845
CACCGTA48850.016.16647531
TCGGAAG52500.015.9459493
ATCGGAA52250.015.7643712
GTAATCT56400.014.20240939
CACGTCT59400.014.16577814
CACACGT60650.013.65127112
CAGTCAC63000.013.60718727
ACGTAAT58700.013.07105337
AATCTCG63200.012.92320741
TACGTAA60050.012.852138536
TAATCTC68450.012.0966640
ACACGTC71250.012.03082413
AGTCACC73200.011.957005528
Produced by FastQC (version 0.11.5)