FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n01_D2W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n01_D2W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26859096
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG46950.031.86819633
GTTTCGG53050.028.24774434
AGTCACG53800.028.22946728
ACGTCTG54650.028.15936515
TTTCGGA58500.025.53914335
TTCGGAA58450.025.48436
ACGTTTC59450.025.43253732
CGTCTGA61550.025.11224716
GTCACGT63750.023.61164929
CTCGTAT52600.023.44094844
TCGGAAT63750.023.40062537
TCGTATG50600.023.12241645
ATCGGAA69900.022.1503892
TCGGAAG73350.021.23083
CACACGT74300.020.9543512
CGGAATC72700.020.33410538
ACACGTC79750.019.80447413
CACGTTT78300.019.48244531
CACGTCT81150.019.35190814
GGAATCT78600.018.46423339
Produced by FastQC (version 0.11.5)