FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n01_D2W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n01_D2W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30346387
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG380900.12551741332501956TruSeq Adapter, Index 20 (97% over 44bp)
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA312250.10289528041674284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG75600.028.51148228
ACGTCTG77700.028.31878515
CTCGTAT71350.027.93950344
CACGTGG77850.027.542431
GGCCTTA77700.027.54001836
CGTCTGA80550.027.48440416
TCGTATG70400.027.3577345
GCCTTAT85750.024.84966537
TATCTCG84800.024.4368741
GTCACGT89100.024.4187529
CACGTCT97800.022.95876314
ACGTGGC95450.022.48725132
TCGGAAG98850.022.3557723
ATCGGAA99100.022.1863062
CACACGT101150.022.10941512
CCTTATC101400.021.10304638
TCACGTG106200.020.48671530
CAGTCAC109500.020.13668827
CTTATCT108250.020.12098939
ACACGTC113600.020.00321813
Produced by FastQC (version 0.11.5)