FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n01_D2W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n01_D2W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16949537
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA223060.13160241486242366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG24050.023.85510315
AGTCACG24550.023.64544528
CGTCTGA25500.022.67509716
CGTGAAA27050.021.4595333
TCGTATG26550.021.35577445
CTCGTAT27500.020.12712144
GTCACGT31950.018.30972129
ACGTGAA32150.018.19528432
CACGTGA31900.018.12655831
AACGATC31050.017.46430838
TGAAACG32800.017.42437235
TCGGAAG35600.016.877963
ATCGGAA36550.016.0702782
CACGTCT36900.016.0356614
AAACGAT36850.015.57040437
CACACGT40200.015.16708112
GAAACGA40150.014.29064736
CAGTCAC42500.014.2410727
ACACGTC45500.014.04314713
TCACGTG43050.013.37969130
Produced by FastQC (version 0.11.5)