FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n01_D2W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n01_D2W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29811680
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402880.13514166259667354No Hit
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA322120.10805160930212587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG39650.022.69844428
ACGTCTG42300.021.59453615
CGTCTGA44450.020.9043416
TCGTATG44550.020.30298245
ACGTCCG45150.020.13230132
CTCGTAT46100.019.3763144
GTCCGCA46500.019.35547334
CCGCACA57050.016.60437436
ATCGGAA57700.015.7958242
TCCGCAC59550.015.79394335
TCGGAAG60450.015.4118473
GTCACGT59800.015.31343229
CACGTCT66200.014.30815814
CACACGT67100.014.14979912
TCACGTC66250.014.09409430
CGCACAT69550.013.36130837
CAGTCAC74300.012.53691927
ACACGTC81050.012.24179713
TCTCGTA78100.011.95579743
TATAGGC51650.011.63108527
Produced by FastQC (version 0.11.5)