FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n01_D2W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n01_D2W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27920638
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGCATCTCGTATG473040.16942306261053203TruSeq Adapter, Index 16 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG79400.032.64285315
CGTCTGA84400.030.73569316
GTCCGCA83750.030.09047536
CTCGTAT74850.029.45839544
TCGTATG73600.028.91932345
TCGGAAG96300.027.2242643
ATCGGAA96750.027.0053252
ACCCGTC96950.026.22434632
CGCATCT95150.026.03567939
CACGTCT100600.026.0323114
TCCGCAT100250.025.02570337
CACACGT108400.024.3252212
TCACCCG106950.024.00425330
CAGTCAC107900.023.834727
ACACGTC116700.022.74934213
CCGTCCG114250.022.72729534
AGTCACC115350.022.56839628
CCCGTCC115800.022.22754533
GTCACCC117100.021.94286529
TCTCGTA103350.021.72675543
Produced by FastQC (version 0.11.5)