FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n01_D2W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n01_D2W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25922472
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG404060.1558724800628582TruSeq Adapter, Index 15 (97% over 40bp)
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA370580.14295704514600305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG105550.031.41902715
CGTCTGA109500.030.265116
CTCGTAT71800.030.08352944
TCGTATG68700.029.73795345
GTCAGAA122550.026.86167536
CAGTCAC125950.026.49267627
TCGGAAG127300.026.233853
ATCGGAA127450.026.0978372
TGTCAGA126700.025.98183335
CACGTCT130300.025.81375114
GTCACAT128150.025.75689329
CACACGT131450.025.43387612
AATCTCG106450.025.36398941
AGTCACA132200.025.13806528
ACACGTC142850.023.92389513
GAATCTC133500.023.78164940
ACATGTC140650.023.5311532
TCACATG144900.023.07441530
CCAGTCA144750.022.9430226
CACATGT146300.022.86872331
Produced by FastQC (version 0.11.5)