FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n01_D2W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n01_D2W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31377657
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG1027030.3273125204982641TruSeq Adapter, Index 14 (97% over 44bp)
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA355100.11316969906325382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTAT153250.036.2952737
CGTATCT152750.036.07458539
TCGTATG135600.036.05608445
CCGTATC156200.035.58053238
AGTTCCG158350.035.42498834
CTCGTAT144950.035.22044444
TTCCGTA165000.033.88788636
GTTCCGT172900.032.3655435
ACGTCTG179300.031.89682615
TATCTCG168650.031.60525741
CGTCTGA181450.031.45688416
GTATCTC192450.028.76146140
TCTCGTA179350.028.55284543
GTCACAG198900.028.2355329
TCACAGT199300.028.18992230
ACAGTTC201050.028.03358832
CACGTCT205300.028.01075614
CAGTCAC202050.027.95127927
CACACGT206900.027.77239412
CACAGTT204800.027.59731331
Produced by FastQC (version 0.11.5)