FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l04_n01_D2W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l04_n01_D2W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24269940
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA317870.13097271769110266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG42650.026.8505815
CGTCTGA46100.024.8899616
CTCGTAT42400.024.35666844
TCGTATG43450.023.56094245
ATCGGAA56450.020.2120292
TCGGAAG57750.019.8732473
CACAGTC58900.019.6730631
AGTCAAC59000.019.29745134
CACGTCT61750.019.12837814
AATCTCG62050.017.73130841
GTCACAG65850.017.52880529
TCACAGT65850.017.5286630
CAGTCAC66500.017.35757827
CACACGT67000.017.32729112
AGTCACA71850.016.221728
TCTCGTA65800.015.93422943
ACACGTC76050.015.79784513
ACAGTCA81250.014.75976232
CCAGTCA79350.014.51832326
CAGTCAA80850.014.38734333
Produced by FastQC (version 0.11.5)