FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18041696
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG300.005121499330.0331631
AGTGTAG5100.029.1206229
TCTCGGT5050.028.9583737
TAGATCT5700.026.44676633
GTAGATC5800.026.381232
GTGTAGA5750.026.2200730
CACTCTT9250.025.565082
GAGTGTA5850.025.38720728
GATCTCG6900.022.49580435
CTCGGTG6700.022.16527438
AGATCTC7300.020.64746534
TGTAGAT7500.020.40202731
GTGTAGG7450.019.3278317
TCGTGTA7650.018.82137715
AGCGGTT6350.018.77446210
TAGGGAA7750.018.57991420
TTCCCTA4250.018.5193278
GTAGGGA7950.018.39504219
TCGGTGG8100.018.0573539
TGTAGGG8100.018.05454318