FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n02_A2W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n02_A2W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27290225
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC33650.011.29778311
GTACTCG59800.010.5432084
CGTGACT26650.010.4787942
TACTCGA59650.010.3761995
AGTACTC69350.09.77395253
CTCGAAT26900.09.46191
TCAGGTA44000.09.4065877
GAATCGA44900.09.3278714
GGAAAAT122500.08.733931
GCGTGAC35400.08.7170491
AAAATCC92200.08.4751773
ATCTAAC12950.08.1639996
TAGAACA47900.08.07802512
AAATCCT93200.07.9245364
CACTCGT49400.07.87790535
AATCGAC33850.07.84583855
AGAACGG157800.07.8345773
GTGACTC36000.07.81911663
TCACTCG51000.07.71911334
GGTAGAA50350.07.68427710
Produced by FastQC (version 0.11.5)