FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n02_A2W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n02_A2W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26756647
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC44750.010.75747811
CAGGTAG56850.08.9418128
TCAGGTA60050.08.8776887
GGAAAAT129250.08.7658511
GGTAGAA56750.08.759810
TAGAACA58950.08.47191512
AAAATCC91800.08.0952983
GAATCGA45550.07.66244754
AAAAATC88150.07.63914252
AGATCAG105250.07.63783554
GAATTGA72300.07.41246844
AGGTAGA68100.07.33270039
AAATCCT93950.07.31016644
CTCGAAT30650.07.27547551
TACGCAA19100.07.1838059
AATTGAC45600.07.1075315
GCCGAAT26500.06.9698691
AGAGATC117650.06.9679522
GAAAAAT168950.06.9593551
GGGGAAT53150.06.95019961
Produced by FastQC (version 0.11.5)