FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n02_A2W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n02_A2W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29604860
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC43100.08.61185311
GGAAAAT128000.07.61943441
AACGGCC120850.07.3191325
AGAACGG172000.07.25310663
AAAAATC109600.07.2123122
AAAATCC90300.07.15709643
GAAAAAT178650.07.11084031
AAGAACG214150.07.05638742
TCAGGTA54800.07.0193347
CGTCCAG137600.06.949206443
CAGGTAG53600.06.8826048
GGGGAAT68650.06.8244491
ATCTAGC45850.06.81960876
GAATCGA46650.06.7574434
GGTAGAA54150.06.72968410
GGACTTA49200.06.727574
AATCGAA57950.06.6801885
ACGGCCG136550.06.67186646
GGGAAAT81650.06.6206381
AAATCCA117050.06.59824944
Produced by FastQC (version 0.11.5)