FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n02_A2W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n02_A2W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26680792
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23250.09.8802062
GTAGAAC32500.09.82877511
AATCGAA53650.09.229445
TCAGGTA46650.08.3419697
GTACTCG48950.08.2341094
CTCGAAT27200.08.1980181
TACTCGA49250.08.13465
TATAAGG27300.07.9146375
CTATAAG26550.07.8874154
AGAACGG172600.07.85427763
AGTACTC60100.07.79362253
AATTGAC42200.07.73353345
GAAAAAT142500.07.713431
GAATCGA45900.07.7019964
GGAAAAT107700.07.65434461
GCGTGAC33050.07.6328921
ATGTCTC110750.07.597849443
GAATTGA71500.07.5897474
ATCGAAC26150.07.31185966
AAGAACG212000.07.27685172
Produced by FastQC (version 0.11.5)