FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n02_A2W5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n02_A2W5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26664501
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC44950.09.90901811
GGAAAAT118100.08.63951
TCAGGTA57750.08.2966117
GGACTTA32750.07.90678024
TAGAACA56750.07.80939612
GGGAAAT71000.07.80399661
GGTAGAA58250.07.76209510
CAGGTAG62100.07.53414968
AATTCAC38950.07.5119055
GAATCGA42250.07.40800484
GAATTGA70250.07.27599954
AATTGAG106300.07.09293175
GACTTAT35000.07.00926835
CGGGTAT10450.06.89721871
GGGGAAT66200.06.87281
ACCTAGG21300.06.864621615
AGGTAGA66050.06.84534749
GAATTCA83950.06.8128084
AGATCAG103200.06.7638684
GGAGAAT90200.06.7421371
Produced by FastQC (version 0.11.5)