FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n02_A2W4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n02_A2W4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29680932
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT27450.010.41953852
GAATCGA50400.09.8288754
GTAGAAC38700.09.47480511
CTCGAAT29000.09.3978221
GCGTGAC36750.08.519171
GGAAAAT139800.08.4906961
AATCGAA64850.08.4681825
GTACTCG59700.07.88285644
AAAATCC100400.07.7382193
AGAACGG180500.07.67279343
TCAGGTA49850.07.6711397
ACGGTAC16750.07.52129220
GAATTGA83000.07.48760034
AATTGAG121900.07.4775895
GTGACTC40400.07.3578033
AAATCCT100800.07.30464124
GGACTTA40800.07.2849314
AAATTGA98000.07.19167234
TACTCGA64250.07.1811015
GCCATGT169200.07.08659117
Produced by FastQC (version 0.11.5)