FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n02_A2W3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n02_A2W3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23135849
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT25800.013.9664542
GTAGAAC31350.011.7677511
GCGTGAC34600.011.3271111
GTGACTC34450.010.9817873
TCAGGTA40250.010.0036247
TAGAACA41000.09.38261312
GTACTCG57000.09.3228724
CTCGAAT22150.09.2536631
GGTAGAA41600.09.13820310
TACTCGA57350.08.9871715
AATTGAC40000.08.8903335
AGTACTC64750.08.5555583
AATCGAA45250.08.5552135
CTAGCAC44550.08.53347914
GGAAAAT93600.08.4465091
AAAAATC64400.08.3928852
GAATCGA38850.08.3461084
TGACTCT49900.08.2577764
GCTCTAG47800.08.2356411
GAATTGA62150.08.2242694
Produced by FastQC (version 0.11.5)