FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n02_A2W2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n02_A2W2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29849530
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC34000.010.519917511
CGTGACT25800.08.9036822
GGAAAAT139800.08.84534551
GTACTCG59050.08.8087794
GAATCGA49800.08.5910824
AAAATCC99600.08.4560293
TACTCGA58700.08.4353735
CTCGAAT30500.08.0496371
GAATTGA81150.07.9914874
TCAGGTA49900.07.97893677
AATCGAA61900.07.7084135
AATCGAC33400.07.68206745
AGTACTC72100.07.589713
AAATCCT103000.07.5860534
AATTGAC51950.07.45182475
ATTGACC50850.07.4319296
TATCCAG66300.07.36658055
GCGTGAC36050.07.24773551
CTCGGAT47600.07.19260641
TAGAACA49500.07.180753712
Produced by FastQC (version 0.11.5)