FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n02_A2W1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n02_A2W1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25146429
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT25100.010.7667562
GTAGAAC32850.010.4087211
GTACTCG48650.08.747464
GCGTGAC34300.08.7337121
GGAAAAT111950.08.6111471
AATCGAA53700.08.5500865
GTGACTC35500.08.49998953
TACTCGA50600.08.4067065
TCAGGTA46250.08.2681967
AAAATCC82550.08.1836323
CTCGAAT26000.08.1432141
GAATCGA41750.07.87407264
GGTAGAA44600.07.76701710
AGTACTC60050.07.68745853
AAAAATC70900.07.65505272
GAATTGA70950.07.64834834
CACTCGA27000.07.58893164
CAGGTAG45900.07.5467818
AATCCGC29600.07.52762655
AAATCCT82900.07.4964834
Produced by FastQC (version 0.11.5)