FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18041696
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG6700.033.56455615
CGTCTGA6950.031.3911816
CACGTCT7250.030.70783614
CACACGT7700.029.49732812
ACACGTC7750.028.4365213
AGACCGA5450.025.62382532
CAGTCAC6950.024.9831527
GAGACCG5900.024.43550931
CCGAGAC5850.024.28411929
CCAGTCA7600.024.0332626
AGCACAC9700.022.9350710
CTCCAGT9500.021.12535524
GCACACG10700.021.00476511
ACCGATC6500.020.75821134
AACTCCA10900.020.25485822
TCGGAAG16050.020.1647993
CGAGACC7450.019.36845430
ATCGGAA17000.019.0387862
CGGAAGA17600.018.3885144
GATCGGA17850.017.8825231
Produced by FastQC (version 0.11.5)