FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n01_A2W9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n01_A2W9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27290225
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA317410.11630904472205708No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288990.10589505949474584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG31450.021.38996315
CGTAATC32650.019.6399138
CCGTACG35200.018.8560233
CGTCTGA36050.018.84782216
CTCGTAT34900.018.1814244
TCGTATG36700.016.00215745
ACCGTAC44000.015.69826332
CACCGTA43650.015.10273631
TCGGAAG49950.013.6950513
CACGTCT51250.013.609035514
ATCGGAA51250.012.9969592
TACGTAA51250.012.95107936
CACACGT55400.012.83325612
ACGTAAT51450.012.46342237
GTAATCT53650.012.24646939
AATCTCG58800.011.51840841
ACACGTC66050.010.934319513
CAGTCAC68900.010.71143227
AGTCACC76750.010.20218228
ACTACGC12150.09.81418719
Produced by FastQC (version 0.11.5)