FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n01_A2W8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n01_A2W8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26756647
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG39050.030.65223333
AGTCACG47100.025.89338728
ACGTCTG48300.025.80597915
GTTTCGG47450.025.1787834
CGTCTGA51800.023.88859616
ACGTTTC50900.023.47185532
TTCGGAA51000.023.29374336
TTTCGGA52500.022.97088835
CTCGTAT44950.021.82463344
GTCACGT56450.021.60457829
TCGTATG44550.020.55595645
CACACGT64700.019.57770512
TCGGAAG65450.019.3217323
ATCGGAA64800.019.3083132
TCGGAAT62850.019.00924337
CACGTTT70050.017.2805631
CACGTCT73700.017.03430614
GGAATCT72600.016.487839
CGGAATC70800.016.46160138
ACACGTC79400.016.2081413
Produced by FastQC (version 0.11.5)