FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n01_A2W7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n01_A2W7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29604860
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG412100.1392001178184933TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG79850.031.16286815
AGTCACG80550.030.44736728
CACGTGG84300.029.0118931
CGTCTGA85800.028.79203216
GTCACGT86500.028.32699829
TCGTATG75250.028.3165545
CTCGTAT77650.028.16577144
GGCCTTA89950.027.18994936
ATCGGAA99550.025.2954792
GCCTTAT96250.025.15310137
ACGTGGC98100.025.04492232
TCGGAAG101400.024.965743
TATCTCG93550.024.70167541
CACGTCT103400.024.43523214
CACACGT110150.023.0195412
TCACGTG108400.022.72853530
ACACGTC113700.022.37996513
CCTTATC112550.021.67024238
CTTATCT113100.021.36682539
TCTCGTA109050.020.44775243
Produced by FastQC (version 0.11.5)