FastQCFastQC Report
Wed 17 Apr 2019
CCWEBACXX_l03_n01_A2W6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCWEBACXX_l03_n01_A2W6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26680792
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGA352210.132008824925437No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320030.11994771369605521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG48600.028.42442115
AGTCACG50200.027.16230828
CGTCTGA52600.026.47673416
TCGTATG51950.025.64060245
CTCGTAT50950.025.393144
CGTGAAA55050.024.56355933
AACGATC55800.023.50765638
ACGTGAA59100.023.22281832
CACGTGA59800.023.10203731
GTCACGT63550.021.56247729
TGAAACG64500.020.82514235
CACGTCT68150.020.7655914
TCGGAAG67150.020.6424373
ATCGGAA68500.020.0721172
CACACGT71050.020.01301412
AAACGAT68750.019.63602337
GAAACGA74150.018.29705236
TCACGTG76300.018.13614830
ACACGTC84250.017.62517713
TCTCGTA80050.016.33080743
Produced by FastQC (version 0.11.5)